rabbit anti ccnb1  (Proteintech)

Journal: World Journal of Gastrointestinal Oncology

Article Title: Baitouweng decoction suppresses growth of esophageal carcinoma cells through miR-495-3p/BUB1/STAT3 axis

doi: 10.4251/wjgo.v16.i7.3193

Figure Lengend Snippet: Protein expression of STAT3, p-STAT3, CCNB1, CDK1, Bax, Caspase3, and Caspase9 in different groups detected using Western blot. A and B: EC9706 cells; C and D: KYSE150 cells. b P < 0.01 compared with normal control.

Article Snippet: The membranes were blocked with 5% skim milk for 1 h at 25 °C and then incubated with primary antibodies against BUB1 (1:1000; Abcam, Cat. No. ab195268), STAT3 (1:1000; Abcam, Cat. No. ab68153), p-STAT3 (1:5000; Abcam, Cat. No. ab76315), CCNB1 (1:1000; Cell Signaling Technology, Cat. No. 12231), CDK1 (1:5000; Abcam, Cat. No. ab133327), Bax (1:1000; Abcam, Cat. No. ab133327), Caspase3 (1:5000; Abcam, Cat. No. ab32351), Caspase9 (1:1000; Abcam, Cat. No. ab32539), and GAPDH (1:2000; Abcam, Cat. No. ab9485) at 4 °C for 12 h. Blots were then incubated with HRP-conjugated secondary antibodies at 25 °C for 60 min. An enhanced chemiluminescence kit (Thermo Fisher Scientific, United States) was used to visualize the blots, and the intensity of the bands was determined using the ImageJ software (version 1.45; National Institutes of Health, MD, United States).

Techniques: Expressing, Western Blot, Control